A Pharmacophore Approach for Novel Inhibitors of Pseudomonas Aeruginosa Exotoxin A
نویسندگان
چکیده
منابع مشابه
PREPARATION AND EVALUATION OF PROPERTIES OF PSEUDOMONAS AERUGINOSA EXOTOXIN A CONJUGATE WITH GOLD NANOPARTICLES
Background & Aims: Pseudomonas aeruginosa is an opportunistic pathogen and most important cause of hospital infections, which causes septicemia and death through various virulence factors such as exotoxin A. In the present study, P. aeruginosa exotoxin A (as a toxic factor) was prepared with the aim of obtaining a new recombinant vaccine candidate and conjugated with gold nanoparticles and its ...
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An enzyme-linked immunosorbent assay (ELISA) is described for Pseudomonas aeruginosa exotoxin A. A double antibody sandwich method was used, employing polyvinyl microtiter plates as the solid phase, a primary coat of monospecific rabbit antitoxin serum, an outer layer composed of a horseradish peroxidase-sheep antitoxin immunoglobulin G conjugate, and an ortho-phenylene-diamine substrate. Absor...
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Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...
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A trypsin-sensitive, heat-labile exotoxin of Pseudomonas aeruginosa strain P-A-103 has been purified by a procedure that includes membrane ultrafiltration, hydroxylapatite chromatography, ion-exchange cellulose chromatography, and gel filtration chromatography. The procedure resulted in the recovery of 48% of the exotoxin with a 40-fold increase in specific activity (micrograms of protein per m...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2013
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.11.2254